Genetic Markers
نویسنده
چکیده
are heterogeneous with respect to molecular weight, buoyant density, and thermal stability. This finding constitutes evidence against unique points of breakage during DNA isolation. The variation in buoyant density suggests a local compositional heterogeneity in the chromosomal region of certain markers. The variation in molecular weight provides an explanation for the results of certain transformation experiments that are otherwise poorly understood. An example of such a result is the observation that acridine orange increases the efficiency of differential thermal inactivation of markers. An explanation of this phenomenon is suggested by the demonstration that acridine orange can decrease the natural intramarker heterogeneity in melting behavior.
منابع مشابه
Evaluation and Comparison of the Efficiency of Different Molecular Markers in Estimating Genetic Distance of Different Persian Oak (Quercus brantii Lindi.) Populations in Lorestan Province, Iran
Estimation of genetic diversity and evaluation of plant germplasm is the most important step in collection and management of plant genetic resources. Also, comparison of different DNA-based genetic markers in diversity evaluation and then advising the most efficient markers is very important. In order to investigate genetic variation among Persian oak (Quercus brantii Lindi.) populations of Lor...
متن کاملGenetic Diversity Evaluation of Lemon balm (Melissa officinalis L.) Ecotypes Using Morphological Traits and Molecular Markers
Genetic diversity of 12 lemon balm ecotypes was evaluated using agronomic traits as well as ISSR and RAPD markers. Results of analysis of variance showed high diversity among the studied ecotypes. Cluster analysis using UPGMA method grouped the ecotypes into three different groups based on the agronomic traits. Twelve ISSR primers created 106 polymorphic bands among the studied ecotypes. An ISS...
متن کاملGenetic Diversity of Bread Wheat (Triticum aestivum L.) Genotypes Using RAPD and ISSR Molecular Markers
The importance of grain cultivation especially wheat is obvious in terms of providing human and animal food and its impact on the economy of human societies. The reduction of genetic diversity in cultivars prevents increasing yields in line with rising demand and consumption. Therefore, it is necessary to improve the compatibility of them and increase their genetic extent. In the current, the g...
متن کاملISSR Markers for Assessing DNA Polymorphism and Genetic Characterization of Cattle, Goat and Sheep Populations
Based on the purpose of conservation planning for native species, sixteen populations of cattle, goat andsheep were analyzed by amplification of genomic DNA using inter-simple sequence repeat (ISSR) markers toestimate of genetic structure. DNA samples of 275 animals were collected to Paccess their genetic content.The polymorphism information content (PIC) values and genetic di...
متن کاملStudy of genetic diversities and relatedness of Iranian citrus genotypes using morphological and molecular markers
Having knowledge about genetic relationships among accessions is necessary for developing breeding strategies to produce improved cultivars. In present study, genetic diversity and inter-relationship among 29 genotypes of citrus were comparatively analyzed using morphological and RAPD markers. Significant variability was observed among citrus genotypes for 61 quantitative and qualitative morpho...
متن کاملAssessment of SCoT and DAMD molecular markers in genetic diversity and species delimitation of three moss species grown in Iran
This study was conducted to assess the efficacy of SCoT and DAMD molecular markers in genetic diversity of three moss species and compare them with ISSR marker. Another objective was to evaluate the suitability of these DNA markers in species identification (delimitation) in three moss species, including Neckera complanata, Homalothecium sericeum and Neckera cris...
متن کامل